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Studies using PPFCs usually target the analysis of cell morphology and alignment in ECs such as porcine aortic ECs [228] and HUVECs [229], and showed that the cells align gradually with the flow direction, morphologically change from a cobblestone aspect to an elongated shape, and alter intercellular space properties such as the increase in junction length

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Nat Commun 14 , 4550 (2023)

However, while this initial hepatic fat accumulation represents a key early event, it is insufficient on its own to drive disease progression