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Dimedone is a cell-permeable reagent that exclusively recognizes and binds to cysteines at their sulfenylated form (4E shows representative N-SIM images of T-cells which revealed a slightly increased global dimedone signal in the cytoplasm and on the cell surface upon SFN treatment

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Tissue proteins (15 g) were separated by electrophoresis on a NuPAGE 4-12% Bis-Tris gel (Invitrogen, Carlsbad, CA, USA) and transferred to a PVDF membrane

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