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For warfarin-resistant VKR screening, the lentiviral Brunello library transduced FIXgla-Fas/HEK293-Cas9 cells were harvested by trypsinization and filtered with 30 m CellTrics filter (Sysmex America, Inc., Lincolnshire, IL) to obtain a well separated single-cell suspension

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Consistent sessions, if recommended, can lead to more sustained benefits