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After the pretreated GC cells were washed once with HBSS, an appropriate 10 mol/L Liperfluo working solution was added, and the mixture was incubated (30 min for 37 C)

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Rahal A, Kumar A, Singh V, Yadav B, Tiwari R, Chakraborty S, Dhama K

This is where the reconstitution ratio pays off