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Materials and Methods Cell Culture 3T3 fibroblasts were cultured in Dulbecco's modified Eagle's medium supplemented with 10% foetal calf serum (Invitrogen, San Diego, CA, USA) and antibiotics (25 U/ml penicillin, 25 g/ml streptomycin, and 0.3 g/ml amphotericin B) (Invitrogen, San Diego, CA, USA) in 5% CO 2 in air at 37C in 25 or 75 cm 2 flasks

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Among the systems of retinal cells to mitigate ROS and manage oxidative stress, a principal one is glutathione (GSH), and it relies on nicotinamide adenine dinucleotide phosphate (NADPH) 1