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The animals were allowed to recover from surgery for several days before use in any experiment, and were fasted overnight prior to the pharmacokinetic experiment

Worked example: 10 mg vial with 2 mL water Concentration: 10 mg divided by 2 mL = 5 mg/mL For a 2.5 mg dose: 2.5 divided by 5 = 0.50 mL = 50 units For a 5 mg dose: 5 divided by 5 = 1.0 mL = 100 units For a 7.5 mg dose: 7.5 divided by 5 = 1.5 mL = 150 units (requires a larger syringe or two draws) Worked example: 30 mg vial with 3 mL water Concentration: 30 mg divided by 3 mL = 10 mg/mL For a 2.5 mg dose: 2.5 divided by 10 = 0.25 mL = 25 units For a 5 mg dose: 5 divided by 10 = 0.50 mL = 50 units For a 10 mg dose: 10 divided by 10 = 1.0 mL = 100 units For a 15 mg dose: 15 divided by 10 = 1.5 mL = 150 units Notice how the 10 mg/mL concentration makes the math cleanest

Yi L, Maier AB, Tao R, et al

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