liposomal glutathione non-alcoholic fatty liver disease Frontiers Glutathione disulfide sensitizes hepatocytes to

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PAL: Phenylalanine ammonia lyase

Sample preparation Tissue sample Wash fresh tissues with PBS for twice, then add 0.1 g of animal/plant tissue into homogenizer (the homogenizer has been rinsed with Reagent I and placed on ice before use)

After pooling the fractions containing pure 3,4- cis -leucocyanidin ( 6 ), MOPSO was added to a final concentration of 10 mM, the pH was set to 6.2 using 5 N NaOH and the pooled fractions were rotary-evaporated to a concentration of 7 mM of 3,4- cis -leucocyanidin ( 6 ), quantified by HPLC-UV (280 nm) with an external calibration curve with 3,4- trans -leucocyanidin ( 14 )