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For transient expression, cells in 12-well plates were typically transfected at roughly 70% confluency using 2.5 l of polyethyleneimine (PEI

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the 5/2 schedule helps mitigate this) Wrist or hand numbness (carpal tunnel-like symptoms from fluid retention) Post-injection flushing Injection site itchiness or mild irritation (normal usually resolves with repeated use) Sleep disturbances (paradoxically, while GH improves sleep long-term, some users experience disruption early on) Tesamorelin Side Effects: Clinical trials showed a small percentage of subjects developed impaired glucose tolerance