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However, reconstitution of Traf6 -/- fibroblasts with TRAF6 C390S was unable to completely rescue P-IB and IFN- mRNA induction in response to HSV-2 and poly(IC) (Figure 5B, C)

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656,657 Major limitations of the use of cell extracts arises from a generally slow turn-on response as a result of inconsistent manufacturing 658,659 and storage 660 of the extracts that does not allow for robust calibration required by a quantitative biosensor