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An increase in intracellular ROS is a major signal that reacts with intracellular iron to produce more active ROS-type hydroxyl radicals (HO) that trigger ferroptosis [81]

Although the clinical effect of l-asparaginase clearly indicates that asparagine is crucial for tumor survival and metastasis 52 , the importance of asparagine beyond protein synthesis and the mechanism by which asparagine supplementation enhances glutamine-associated metabolism are less well understood

Bioavailability can be determined from the ratio of the areas under the concentrationtime curves for an identical bolus dose given both orally and intravenously (Figure 2.1)

For evaluation of in vivo tumor growth, a total of 6 10 5 cells (resuspended in 50 L DMEM medium and 50 L Matrigel) were subcutaneously injected into the flank of mice