Exonuclease III, 25,000 U @ 200 U/uL OverExpress C41(DE3) ChIP-seq and FFPE and cell

ChIP-seq and FFPE and cell free DNA samples

Choice of efficiency:

T4 DNA ligase was loaded at 5 units (Lane 2) and 10 units (Lane 3)

Academic Press

Exonuclease III, 25,000 U @ 200 U/uL OverExpress C41(DE3) ChIP-seq and FFPE and cellDigest duplex DNA in a 3 to 5 direction from a nick, blunt end, or 3 recessed end, producing stretches of ssDNA. Produce stretches of ssDNA in dsDNA templates containing nicks, blunt ends or recessed 3' ends Protect protruding 3' ends becuase this exonuclease will not start digestion at protruding 3' overhangs Applications Production of intermediates for site directed mutagenesis. 1 3 Production of strand specific radiolabeled probes. 4 Exonuclease