Quantitative Precipitin Assay size:For 6 groups of 4-5 or 24-30 students. A centrifugal propeller consiting of

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500 µg per ml of pHC79 cosmid DNA was incubated for 15 minutes at 22°C in TE buffer (25 mM Tris (pH 8

Quantitative Precipitin Assay size:For 6 groups of 4-5 or 24-30 students. A centrifugal propeller consiting ofThe Quantitative Precipitin Technique is a simple technique that is routinely used in the analysis of antibody and antigen interactions and for the estimation of the antibody or antigen content in a sample. The technique is based on the interaction of antibody and antigen to form a large protein complex that in certain solutions (buffer) will result in precipitation. Students undertake a simple experiment to determine the zone of equivalence of an