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The eluted compounds were detected using LCMS-8030As LIQUID CHROMATOGRAPH MASS SPECTROMETER-Triple Quad MS (Waters Corporation, Milford, MA, USA) supplied with an electrospray ionization source (ESI) running in positive ionization mode to generate [M + H] + ion at m/z 162.23, 176.21 for nicotine and cotinine, respectively

Cigarettes are placed into four categories as per the Centers for Disease Control and Prevention (CDC) in the US, which are as follows

Major manufacturers include Swedish Match (ZYN producer), British American Tobacco (VELO), and Philip Morris International

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