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Materials and methods Genetic materials The backcross breeding method was used to create various combinations of the nic1 and nic2 alleles derived from LAFC53 [30], and the mutated myc2a allele derived from TI 313 [1], in the genetic background of elite flue-cured tobacco cultivar K326. During the backcrossing effort, plants carrying the 5 bp myc2a mutation were identified via a cleaved amplified polymorphic sequence (CAPS) marker assay

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Most of the women said they didn't smoke, but about 13 percent of the women were daily smokers and another 25 percent admitted to occasional smoking during pregnancy

The disease process involves an inappropriate relaxation of the lower esophageal sphincter Lower Esophageal Sphincter Esophagus: Anatomy