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She worked as a translator, jogged daily, read voraciously and continued to write and speak out against oppression

Nuclear protein extraction HCE-T cells cultured in 60 mm dishes (Corning Inc.) were rinsed twice with ice-cold PBS and then harvested by scraping in the presence of 500 l of hypotonic buffer [20 mM TrisHCl (pH 7.4), 10 mM NaCl, and 3 mM MgCl 2 ] containing a protease inhibitor cocktail as well as phosphatase inhibitor cocktails 2 and 3 (Sigma-Aldrich)

Biopsies These are tissue samples from lung nodules, lung masses, lymph nodes within the chest, and other lesions within or outside the chest