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All pathway enrichment analyses of differentially abundant proteins in EV samples were performed using the DAVID bioinformatics database ( Soft agar colony forming assay The ability of BCEVs derived from TCCSUP cells treated with UEL, MEL, or CSE to induce the transformation of SV-HUC cells was assessed by subjecting these cells to long-term BCEV treatment and then performing a soft agar colony forming assay, in vitro tumorigenic assay, to test for anchorage-independent growth 40

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Just the cigarettes. Hed started smoking when he was a teenager and then blithely fumed his way through World War II while stationed in the Aleutian Islands, lighting up free cartons of Lucky Strikes