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That we did not observe this phenomenon in CLCNt2 -RNAi plants might be attributable to: (1) the RNAi-mediated suppression of CLCNt2 being less effective in the root than in the leaf and/or (2) the low levels of CLCNt2 protein still produced in knockdown plants, as opposed to that in KO plants, is sufficient to meet the needs for vacuolar sequestration of nitrate within root tissue