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The increasing demand for tobacco in Europe fueled the transatlantic trade, leading to the expansion of plantations and the use of enslaved labor

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PCR was performed using the primers ( PparoA 1 optimized F 5-ATGGCGCAAGTTAGCAGAATC-3 and PparoA 1 optimized R 1 5-TCATGAGAA GTTGAATTGATG-3), resulting in 1521 bp amplified DNA product ( AtCTP - PparoA 1 optimized )

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