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Most of the harshness people associate with "the smoke being too hot" is actually these particles, not the smoke temperature itself

You're not watching this video from the 1600s, presumably, so we probably don't need to tell you that smoking tobacco is actually really, really bad for you

doi: 10.18001/TRS.3.1.9 7

On the day of capture, cells were quick thawed, washed with 1 PBS (no BSA) and counted before proceeding with WaferGen capture according to the manufacturers instructions with the following modifications: 5 or 10 10 4 cells were stained per sample, single-cell candidates were confirmed by manual visual triage