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Primary antibodies against HIF-1 (610958, BD Biosciences, Franklin Lakes, NJ), hydroxy-HIF-1 (3434, Cell Signaling Technology, Danvers, MA), HIF-2 (NB100-122, Novus Biologicals, Littleton, CO), TOM20 (11802-1-AP, Proteintech Group, Rosemont, IL), HSP60 (15282-1-AP, Proteintech Group), and Tubulin (sc-8035, Santa Cruz Biotechnology, Dallas, TX) were diluted in blocking buffer and incubated overnight at 4 C

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Graphic warnings help The images and warnings are found to be effective in reducing smoking when they are combined with higher taxes, enforcement of smoke-free public places, and restrictions on advertising, Bar-Zeev said