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In order to determine whether Smoothol flavor is truly influencing REDD1, we repeated the experiment by exposing the BEAS-2B cells directly to 1% e-liquids, and the results showed that Smoothol flavor did increase REDD1 protein levels by 1.2 in no-nicotine and 1.4-fold in 6 mg/mL nicotine e-liquids (Figure 4 B)

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