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Nicotine also induced osteoblast apoptosis by both increasing the accumulation of H 2 O 2 and inhibiting nuclear factor kappa-light-chain-enhancer of activated B cells (NF-KB) activation, as evidenced by reduced nuclear levels of NF-kB p65, which subsequently resulted in upregulation of B-cell lymphoma 2 (Bcl-2), bcl-2-associated X protein (BAX) and caspase-3 and in-turn apoptosis [26]

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