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FIGURE 5 Among these co-enriched pathways, a total of seven metabolic pathways are closely related to the formation of cigar quality, including amino acid biosynthesis, aromatic amino acid metabolic (tryptophan, tyrosine and phenylalanine), starch and sugar metabolic, carbon metabolic, and nicotinate and nicotinamide metabolism

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Finally, a cash crop to export had been identified, and plantations and new outposts sprung up, initially both upriver and downriver along the navigable portion of the James River, and thereafter along the other rivers and waterways of the area

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