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To accomplish this, reagents and working standards were prepared according to the instructions provided by the ELISA kit (D6050, DTA00D, R&D systems, USA) and kept the room temperature for 20 min

Further efforts are needed to identify the major cause or causes of lung cancers arising in never smokers before successful strategies for prevention, early diagnosis and novel therapies can be implemented

Smokers who littered their cigarette were on average 9.45 m away from the nearest ashtray [3] and littering evidently increased from distances of 5 m and more [5]

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