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The focus was on coding variants that could alter the protein sequence, such as protein-truncating variants (i.e., nonsense, frameshift, or splice-site variants that abolish a canonical splice-site [-2 or +2 bases from exon boundary]) and missense variants

Varney had filmed an anti-smoking public service announcement as Ernest in the 1980s, though it is often mistakenly assumed to have been filmed after his diagnosis

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