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The tissue inserts were transferred into 1 mL of fresh culture medium, which bathes the basolateral side of the tissue, and exposed to the apical surface to DA vapor for 1 h at 37 C (+5% CO 2 ) using vapor cups (MatTek)

Synthetic nicotine, on the other hand, is produced in a controlled lab environment

Oral nicotine products, particularly the nicotine pouch brand of ZYN, has received significant media attention and congressional attention

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