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doi: 10.1080/21655979.2021.1950259 153 ChenYShenJYuanMLiHLiYZhengSet al

(2002a)

Metabolic enzymes of amino acids The level of amino acids is influenced by various factors, especially enzyme activity

Trypan blue for cell death and DAB staining for H 2 O 2 accumulation Detached leaves were immersed in trypan blue staining solution (2.5 mg ml 1 trypan blue dissolved in lactophenol:lactic acid:glycerol:liquid phenol:ddH 2 O at 1:1:1:1:1) for 30 min