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Description

A double labeling in situ hybridization analysis

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qPCR was performed according to the protocol consisting of 40 cycles: polymerase activation and DNA denaturation 95 C (3 min), denaturation 95 C (15 s), annealing 60.5 C (15 s), extension and plate reading at 72 C (40 s)

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Allicin equivalence is not a perfect measure of allicin alone because other sulfur containing compounds also break down to AMS, many of which have similar therapeutic effects as allicin

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