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The Ki67 channel was held to a consistent threshold within each replicate and the Ki67 area fraction for each nucleus was recorded using the ROI manager as done with the pNRF2

Several studies suggest creatine supplementation attenuates cognitive decline associated with sleep deprivation, a particularly relevant finding given perimenopausal sleep disruption [24]

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GLAST-Cre-Ai9 mice were orally gavaged with peanut oil (control) or 1 mg tamoxifen (TMX) (dissolved in peanut oil) every alternate day for 3 days