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The server revealed that the wild-type residues of R153C and R233C are more hydrophobic than the mutant residues, and these variations in size and hydrophobicity disrupt the H-bond interactions with the adjacent molecules due to loss of hydrophobic interactions in the core of the protein

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The method of any one of embodiments 102-106, wherein the composition has an osmolarity from about 100 mOsm/L to about 1000 mOsm/L