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List of standards used for the identification of APAP metabolites and the 15 labeled internal standards (IS) spiked in samples for the non-targeted analyses and their respective suppliers

Insofar as aminoacrylate takes seconds to many minutes to be nonenzymatically converted to pyruvate and ammonia, the possibility exists that proteins and other macromolecules in the vicinity of the aminoacrylate-generating enzymes will be severely damaged. It is of considerable interest, therefore, that nature has evolved enzymes (RidA proteins) that are widely distributed and that considerably accelerate the rate of conversion of aminoacrylate to pyruvate and ammonia, thereby lessening the impact of enzyme-generated aminoacrylate (Ernst et al., 2014, Niehaus et al., 2014, Niehaus et al., 2015)

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This does not mean it would be ineffective for every patient but it does raise questions about expectations