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The expression levels were normalized to the expression of the housekeeping gene RSP-9 (40S ribosomal protein S9) and then quantified using the 2 Ct method according to Livak and Smittgen 62 using StepOne v2.3 software (Applied Biosystems)

For surface sterilization, seeds were soaked in 1% sodium hypochlorite for 15 min, followed by five thorough washes with double-distilled water at intervals

Key Benefits of Glutathione for Detox and Skin Health Key Benefits of Glutathione for Detox and Skin Health Glutathione is often referred to as the body's master antioxidant

doi: 10.1016/j.cub.2009.07.072 32 DebrayFGDrouinEHerzogDLortieALambertMGarelLet al