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At the cellular level, mitochondria play a central role in energy metabolism, primarily through oxidative phosphorylation, which generates ATP [8]

Liquid-liquid extraction, either with acetonitrile based on QuEChERS (Quick, Easy, Cheap, Effective, Rugged, Safe) protocols or by salting-out assisted extraction with ethyl acetate are other commonly used sample preparation techniques used also for large cohorts (Njumbe Ediage et al

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