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Ca 2+ combines with calmodulin (CaM) -binding domain that activates the glutamate decarboxylase (GAD) enzyme for the conversion of glutamate into GABA

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Western blotting HTMCs were grown in the above-mentioned TMC medium in five 12-well plates at a density of 5 10 4 /ml for 24 h, and the medium was replaced with a new one, containing 1 l of water (negative control), TGF-1 (20 g/ml) or glucaric acid (10 mM) for 10 min

A presumptive diagnosis of Gilbert syndrome can be made in an otherwise well patient who has unconjugated hyperbilirubinemia, normal liver enzyme values, and a normal concentration of hemoglobin (to exclude hemolysis)