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Following the end of each daily IH exposure, all cages were transported back into housing racks during the dark cycle

As a central energy sensor, AMPK extends lifespan by promoting autophagy via mTOR inhibition and ULK1 activation, enhancing mitochondrial function through PGC-1/SIRT1 signaling and improving the NAD + /NADH balance, suppressing inflammatory responses via NF-B inhibition, and modulating stress resistance via the FOXO3/p53 pathways, thereby linking metabolic regulation to aging suppression 41

Avoiding excessive salt, alcohol, and caffeine further supports healing, while maintaining stable blood pressure and minimizing fluid retention

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