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Introduction Diabetes mellitus (DM) is a complex metabolic disorder which adversely affects multiple physiological systems including the central nervous system and cardiovascular system, and the disease is associated with altered memory function, cardiac stress, endothelial dysfunction and neuropathy (Islam et al., 2017

For instance, a study by Grace et al., on lambs receiving a single 2 mg subcutaneous B12 injection demonstrated significant increases in serum and liver B12 concentrations for up to 24 days compared to untreated animals (2)

Further research is needed to reveal the exact metabolic functions of GST isoenzymes in infected plants and to understand their contribution to disease resistance

It is particularly useful in models exploring cellular development, tissue signaling, and coordinated metabolic regulation, especially in environments designed to evaluate long-acting pathway activation