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The percentage of cell viability was expressed as a percentage relative to the untreated control, and the EC 50 was calculated

4.2.3 Regulation of ferroptosis by iron metabolism Ferroptosis can be inhibited by reducing iron uptake and using iron chelators

These findings illustrate the dual role of S1P: it enhances oxygen release under controlled conditions but, when dysregulated, contributes to red blood cell dysfunction and disease progression

They were maintained in IMDM medium (Invitrogen), and DMEM medium (Invitrogen) correspondingly, supplemented with 10% fetal bovine serum (FBS) (S711-001S, Lonsera) and 1% Penicillin-Streptomycin (SV30010, HyClone)