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Preyat N, Leo O (2016) Reassessing the role of NAD as a prosurvival factor

For immunofluorescence staining, slides were deparaffinized, hydrated, blocked with 10% heat-inactivated horse serum (HIHS) and 0.3% Triton X-100 in PBS, incubated with primary antibody in 1% HIHS and PBS overnight at 4 C and secondary antibody in PBS for 3060 min at room temperature 6

In one embodiment of the otic formulations or compositions described herein, the otic formulation or composition is provided as a suspension formulation composition, also referred to herein as auris-acceptable suspension formulation or composition, auris suspension formulations or compositions or variations thereof