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The tissue was mechanically minced with sterile scissors and enzymatically digested in collagenase type I solution (0.1 mg/mL) (SCR103, Merck Millipore, Darmstadt, Germany) for 40 min in a CO incubator (37 C, 5% CO2 and 95% humidity) (BINDER GmbH, CBF170, Tuttlingen, Germany)

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One reviewer stated that BPC-157 Pure healed esophageal damage from acid reflux when conventional treatments had failed