Affiliation: 1 Department of Biochemistry, Co-Operative Institute of Health Sciences, Kannur 670105, Kerala, India ORCID: Affiliation: 2 Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Malaya, Kuala Lumpur 50603, Malaysia ORCID: Affiliation: 3 Division of Animal Physiology, ICAR-National Institute of Animal Nutrition and Physiology, Bengaluru 560030, Karnataka, India ORCID: Affiliation: 4 Department of Biochemistry, Royal Dental College, Palakkad 679536, Kerala, India ORCID: Affiliation: 5 Department of Biotechnology, University of Calicut, Thenhipalam 673635, Kerala, India ORCID: Affiliation: 6 Division of Biochemistry, Malabar Cancer Centre, Kannur 670103, Kerala, India Email: [email protected] ORCID: Explor Drug Sci
For the GSSG assay, GSH was masked by adding 20 L of 2-vinylpyridine to the neutralized supernatant, whereas 20 L of water was added for the total GSH assay
Therefore, avidin can be used to selectively mask free biotin in cell lysates or cell culture supernatants, whereas the Strep-tagII or Twin-Strep-tag at the target protein remain accessible
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