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In the atherosclerotic lesions, the primary source of chronic inflammatory responses is represented by lipid-laden foam cells caused by excessive lipid accumulation, ROS generation, intramural retention of oxidized LDLs (oxLDLs), and minimally oxLDLs, with many of these responses mediated by toll-like receptor-4 (TLR-4, by recognition and assembly of multi-molecular complexes, reviewed elsewhere) (54)

While high plasma homocysteine is widely recognized as a cardiovascular disease risk factor, individuals with low homocysteine may also be at risk

After the continuous stirring for 6 h at room temperature, the solids were centrifuged (6000 g for 5 min), and washed with methanol three times