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the membranes were then washed several times with water and TBS (PBS, 0.05% Tween-20), a 1:5000 dilution of horseradish-peroxidase conjugated goat anti-rabbit antiserum was added, and the membranes further incubated for 1 h at room temperature

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On the same note, the growth factor therapies are usually not very effective because they are quickly degraded, they show limited penetration into ischemic tissue and the diabetic cells do not respond to growth factor signalling in the most effective way

Mehrvarz Sarshekeh A, Loree J, Lima Pereira AA, Raghav KPS, Lam M, Advani SM, Davis JS, Dasari A, Morris VK, Menter D, Eng C, Shaw KR, Broaddus R, Routbort M, Luthra R, Maru DM, Overman MJ, Meric-Bernstam F, Kopetz S