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Hippocampal neurons were maintained in Neurobasal A media from Invitrogen (Carlsbad, Calif.) supplemented with B27 from Invitrogen, 0.5 mM L-glutamine, and 5 mM cytosine-D-arabinofuranoside from Sigma added at 2 days in vitro

Several factors influence how long MIC B12 remains active in the body: Individual metabolic rate Frequency of injection administration Overall health status Lifestyle factors Current nutritional status The body processes these water-soluble compounds efficiently, naturally eliminating any excess amounts through urination [11]