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These tools integrated into Defined Approaches (DAs) 249 allowing data from individual assays to be combined and utilized for chemical classification (either binary (sensitizer/nonsensitizer) or the United Nations Globally Harmonized System of Classification and Labeling of Chemicals (UN GHS) hazard subcategories 1A (strong sensitizer) or 1B (moderate or weak sensitizer))

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Subsequently, the cells were exposed to HT treatment for 48 h, after which the complete medium was replaced, and the cells continued to incubate for 5 days