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Vitamin D did not exhibit a ROS scavenger effect, and the antioxidant effect is presumably mediated via its receptor whose presence was confirmed in the varicose venous samples

The peptide has a uniquely high affinity for copper ions, forming a stable chelate with copper(II) that is more exchangeable than the copper bound to albumin, allowing efficient copper delivery to tissues

Zinc, selenium, manganese, vitamin C and vitamin E contribute to the protection of cells from oxidative stress

However, the convenience of a single morning dose means most researchers prefer once-daily administration, and the clinical data supporting split dosing specifically for AOD 9604 is limited