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While enzymatic kits measuring intracellular GSH and glutathione disulfide (GSSG) are commonly employed in cell line development workflows, comprehensive LC-MS-based methods enhance redox analysis to include the amino acid components of GSH (cysteine, glycine, and glutamate), the GSH/GSSG ratio, and other markers and mitigators of ROS, both inside and outside the cell

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These results demonstrate that the low molecular weight bioactive peptides contained in CPNS, such as Gly-Pro and Pro-Hyp, exert positive effects on skin hydration, elasticity, desquamation, and wrinkling

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