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Longitudinal registration was processed with Inscopix software and a custom MATLAB script to track the same neurons across sessions as described 71,72 ( Quantification and statistical analysis OriginPro 2017 software (OriginLab Corporation, USA) and GraphPad Prism 8 (GraphPad Software, Inc., USA) were used for the statistical analyses and graphing

The lysates were collected and centrifuged at 12,000 g for 20 min to obtain the supernatant