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Once the samples reached confluency the cells were subcultured and all tendon fibroblasts between passages 2 and 4 that had a proper growth rate and normal fibroblast shape were used in the experimentation process

Step 1: Exposure to UV radiation generates Reactive Oxygen Species (ROS) [3] Step 2: High levels of ROS induce oxidative stress, which leads to Tyrosinase activation, the enzyme responsible for melanin synthesis

However, with high or chronic levels of oxidative stress this effect gets much larger and the loss of cysteines is quite large

Chakraborty S, Filippi CG, Wong T, Ray A, Fralin S, Tsiouris AJ, et al