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Taken together, disturbed Li homeostasis may contribute to early AD biology (potentially via GSK3/-catenin signaling and microglial/myelin vulnerability), but lithium is not an established AD therapy

Blots were first incubated with 5% BSA and 0.1% Tween in Tris-buffered saline (TBS) for 1 h at room temperature, and then with either polyclonal rabbit primary antibody against Orai1 (1:1,000, rabbit Sigma-Aldrich, O8264), STIM2 (1:2,000, rabbit Sigma-Aldrich, S8572) or monoclonal mouse actin (1:100, mouse Sigma-Aldrich, A2066) at 4C overnight

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S Afr J Bot 131:335341 Huang T, Jander G, de Vos M (2011) Non-protein amino acids in plant defense against insect herbivores: representative cases and opportunities for further functional analysis