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According to earlier research, Failure to adhere to these behaviours increases the likelihood of adverse pregnancy outcomes, including but not limited to preterm delivery, hypertension, low birth weight, and neurodevelopmental complications like fetal alcohol spectrum discord (Garca et al., 2020)

Gastroenterology 117:884892 Rachmilewitz D, Karmeli F, Okon E, Bursztyn M (1995) Experimental colitis is ameliorated by inhibition of nitric oxide synthase activity

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To investigate this, we designed two pairs of RTqPCR primers, one for the coding sequence of the N-terminal extension and the other for the adjacent signal peptide sequence